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![hnRNP U/p120 Monoclonal Antibody [3G6]: IHC](http://immuquest.com/cdn/shop/products/1360144925-91300100_1000x1000.jpg?v=1563518759)
![hnRNP U/p120 Monoclonal Antibody [3G6]: IHC](http://immuquest.com/cdn/shop/products/1360144925-91300100_{width}x.jpg?v=1563518759)
Current Image Legend: hnRNP U/p120 Monoclonal Antibody [3G6]: IHC
hnRNP U/p120 Monoclonal Antibody [3G6]
Catalogue Number: IQ266
Catalogue Number: IQ210
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Datasheet
Download Variant Datasheet
| Primary Description: | Mouse Anti-hnRNP U [3G6] |
| Primary Description: | Mouse Anti-hnRNP U [3G6] |
| Target Antigen: | hnRNP U/p120 |
| Target Antigen: | hnRNP U/p120 |
| Catalogue Number: | IQ266 |
| Catalogue Number: | IQ210 |
| Quantity: | 0.05mg |
| Quantity: | 0.1mg |
| Concentration: | 1mg/ml |
| Concentration: | 1mg/ml |
| Clone: | 3G6 |
| Clone: | 3G6 |
| Host: | Mouse |
| Host: | Mouse |
| Isotype: | IgG1 |
| Isotype: | IgG1 |
| Myeloma/Fusion Partners: | SP2/0 |
| Myeloma/Fusion Partners: | SP2/0 |
| Immunogen: | RNPs eluted from an oligo (dt) cellulose column |
| Immunogen: | RNPs eluted from an oligo (dt) cellulose column |
| Species Reactivity: | Mouse, Human, Monkey. Does not react with Chicken, Lizard |
| Species Reactivity: | Mouse, Human, Monkey. Does not react with Chicken, Lizard |
| Purification: | Protein A |
| Purification: | Protein A |
| Format: | Purified antibody (from supernatant) containing PBS 0.1% sodium azide; |
| Format: | Purified antibody (from supernatant) containing PBS 0.1% sodium azide; |
| Applications: | ELISA, WB, IP, ICC/IF, IHC-P, Flow Cyt |
| Applications: | ELISA, WB, IP, ICC/IF, IHC-P, Flow Cyt |
| Dilutions: | Optimal antibody dilution should be determined by titration, however as a guideline try;WB: Use at an assay dependent dilution. Detects a band of approximately 97 kDa (predicted molecular weight: 120 kDa).ICC/IFICC/IF: 1/100. Flow Cyt: Use 1µg for 106 cells. |
| Dilutions: | Optimal antibody dilution should be determined by titration, however as a guideline try;WB: Use at an assay dependent dilution. Detects a band of approximately 97 kDa (predicted molecular weight: 120 kDa).ICC/IFICC/IF: 1/100. Flow Cyt: Use 1µg for 106 cells. |
| Also Known As: | Heterogeneous nuclear ribonucleoprotein U antibody;hnRNP U protein antibody;HNRNPU antibody;hnRNPUprotein antibody;HNRPU antibody;p120 antibody;p120 nuclear protein antibody;pp120 antibody;SAF Aantibody;SAF-A antibody;SAFA antibody;Scaffold attachment factor A antibody;U21.1antibody |
| Also Known As: | Heterogeneous nuclear ribonucleoprotein U antibody;hnRNP U protein antibody;HNRNPU antibody;hnRNPUprotein antibody;HNRPU antibody;p120 antibody;p120 nuclear protein antibody;pp120 antibody;SAF Aantibody;SAF-A antibody;SAFA antibody;Scaffold attachment factor A antibody;U21.1antibody |
| Pubmed ID(s): | 30498093, 30799487, 29273625, 28546514, 28622508, 28221134, 28539331, 28475895, 27041735, 27068814, 25568207, 25986610, 24362020, 23830498093, 30799487, 29273625, 28546514, 28622508, 28221134, 28539331, 28475895, 27041735, 27068814, 25568207, 25986610, 24362020, 23825951, 21242313, 21646346, 21471000, 21951848, 19808671, 19066202, 16757475, 16001081, 15835916, 6204191 |
| Pubmed ID(s): | 30498093, 30799487, 29273625, 28546514, 28622508, 28221134, 28539331, 28475895, 27041735, 27068814, 25568207, 25986610, 24362020, 23830498093, 30799487, 29273625, 28546514, 28622508, 28221134, 28539331, 28475895, 27041735, 27068814, 25568207, 25986610, 24362020, 23825951, 21242313, 21646346, 21471000, 21951848, 19808671, 19066202, 16757475, 16001081, 15835916, 6204191 |
| Entrez Gene ID(s): | 3192, 51810 |
| Entrez Gene ID(s): | 3192, 51810 |
| SwissProt ID(s): | Q00839, Q8VEK3 |
| SwissProt ID(s): | Q00839, Q8VEK3 |
| Omim ID(s): | 602869 |
| Omim ID(s): | 602869 |
Citations
| Citation Count: | 24 |
| Citations: | Sun WL et al. Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410. Biosci Rep 39:N/A (2019). Alfano L et al. Depletion of the RNA binding protein HNRNPD impairs homologous recombination by inhibiting DNA-end resection and inducing R-loop accumulation. Nucleic Acids Res N/A:N/A (2019). Fan H et al. The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes. Genome Res 28:192-202 (2018). Ridings-Figueroa R et al. The nuclear matrix protein CIZ1 facilitates localization of Xist RNA to the inactive X-chromosome territory. Genes Dev 31:876-888 (2017). ICC/IF ; Mouse . Nozawa RS et al. SAF-A Regulates Interphase Chromosome Structure through Oligomerization with Chromatin-Associated RNAs. Cell 169:1214-1227.e18 (2017). Capitanio JS et al. Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9. Elife 6:N/A (2017). Liu K et al. Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p. Biosci Rep 37:N/A (2017). Xing YH et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell 169:664-678.e16 (2017). Huang X et al. Protein phosphatase 4 plays dual roles during cell proliferation. Cell Prolif 49:219-35 (2016). Meininger I et al. Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells. Nat Commun 7:11292 (2016). Mouse . Majerciak V et al. Stability of structured Kaposi's sarcoma-associated herpesvirus ORF57 protein is regulated by protein phosphorylation and homodimerization. J Virol 89:3256-74 (2015). Douglas P et al. Phosphorylation of SAF-A/hnRNP-U Serine 59 by Polo-Like Kinase 1 Is Required for Mitosis. Mol Cell Biol 35:2699-713 (2015). Workman E et al. U1A regulates 3' processing of the survival motor neuron mRNA. J Biol Chem 289:3703-12 (2014). Tsai PL et al. Cellular RNA binding proteins NS1-BP and hnRNP K regulate influenza A virus RNA splicing. PLoS Pathog 9:e1003460 (2013). IP ; Human . Ma N et al. The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. J Cell Sci 124:394-404 (2011) Tserel L et al. MicroRNA Expression Profiles of Human Blood Monocyte-derived Dendritic Cells and Macrophages Reveal miR-511 as Putative Positive Regulator of Toll-like Receptor 4. J Biol Chem 286:26487-95 (2011). WB ; Human Raju CS et al. In neurons, activity-dependent association of dendritically transported mRNA transcripts with the transacting factor CBF-A is mediated by A2RE/RTS elements. Mol Biol Cell 22:1864-77 (2011). WB ; Mouse Pare JM et al. MicroRNA-binding is required for recruitment of human Argonaute 2 to stress granules and P-bodies. Biochem Biophys Res Commun : (2011). WB ; Human Ma N et al. The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. J Cell Sci 124:394-404 (2011). Tserel L et al. MicroRNA Expression Profiles of Human Blood Monocyte-derived Dendritic Cells and Macrophages Reveal miR-511 as Putative Positive Regulator of Toll-like Receptor 4. J Biol Chem 286:26487-95 (2011). WB ; Human . Raju CS et al. In neurons, activity-dependent association of dendritically transported mRNA transcripts with the transacting factor CBF-A is mediated by A2RE/RTS elements. Mol Biol Cell 22:1864-77 (2011). WB ; Mouse . Pare JM et al. MicroRNA-binding is required for recruitment of human Argonaute 2 to stress granules and P-bodies. Biochem Biophys Res Commun : (2011). WB ; Human . Hadian K et al. Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein. J Biol Chem 284:33384-91 (2009). WB ; Human Hadian K et al. Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein. J Biol Chem 284:33384-91 (2009). WB ; Human . Pacheco A et al. A novel role for Gemin5 in mRNA translation. Nucleic Acids Res. 2009 Feb;37(2):582-90. doi: 10.1093/nar/gkn979. Epub 2008 Dec 9. WB, IP; Human Uchimura Y et al. Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. J Biol Chem. 2006 Aug 11;281(32):23180-90. Epub 2006 Jun 5. ICC/IF; Human HnRNP L represses exon splicing via a regulated exonic splicing silencer. Rothrock CR et al. EMBO J. 2005 Aug 3;24(15):2792-802. Epub 2005 Jul 7 Ahmad N et al. Kruppel-like factor 2 transcriptional regulation involves heterogeneous nuclear ribonucleoproteins and acetyltransferases. Biochemistry. 2005 Apr 26;44(16):6276-85. ChIP, EMSA; Mouse Dreyfuss G et al. Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies. Mol Cell Biol 4:1104-14 (1984) Dreyfuss G et al. Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies. Mol Cell Biol 4:1104-14 (1984). |
Protocols
Immunofluorescence protocol - Formaldehyde fixation
- Collect cells from T.c.unit and remove media from petri dish using suction.
- Wash with 1x PBS and remove.
- Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperature on an orbital shaker.
- Remove PFA and incubate in 0.5% Triton X-IOO in 1x PBS for 5 minutes at room temperature.
- Prepare blocking reagent, this is also the antibody diluent.
- Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker.
- Block with 1 % NCS and 1x PBS for 30 minutes at room temperature.
- Prepare primary antibodies (50?l/coverslip) and moist staining chambers.
- Wash cells 2x with lx PBS at room temperature and air dry briefly.
- Incubate with primary antibody for 1 hr at room temperature in the dark in staining chambers. During this time prepare the secondary antibody.
- Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)
- Incubate with secondary antibody for 1 hour at room temperature in the dark in staining chambers.
- Wash cells 5x with 1x PBS.
- Mount in Dapi.
Solutions (prepare fresh the same day of staining).
- * 1x Phosphate buffered saline.
- * Blocking reagent: 1% NCS in 1x PBS (use fresh l0x PBS).
- * Fixation solution: 3.5% Para formaldehyde.
1.75g PFA in 20 ml d.H20 plus 5 drops 1M NaOH. Stir on a hot plate at 50-60°C until dissolved. Add 4 drops IN HCI and check pH indicator strip. PH should be 7.4. Complete volume with d.H20 to 25ml and add 25ml 2xPBS. Check pH before adding to cover slips.
Immunofluorescence protocol - Methanol/acetone fixation
- Collect cells from T.C.unit and remove media from petri dish using suction.
- Wash with 1x PBS and remove.
- Fix cells with cold methanol: acetone 1: 1 for 10 minutes on ice.
- Prepare blocking reagent, this is also the diluent for the antibodies.
- Remove fixative and wash cells 3x with Ix PBS at RT, for 4 minutes/wash on orbital shaker.
- Block with 1% NCS and Ix PBS for 30 minutes at RT.
- Prepare primary antibodies (50?l/coverslip) and moist staining chambers.
- Wash cells 2x with 1 x PBS at RT and air dry for approximately 7 minutes.
- Incubate with primary antibody for 1 hr at RT in the dark in staining chambers. During this time prepare secondary antibody.
- Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)
- Incubate with secondary antibody for 1 hr at R T in the dark in staining chambers.
- Wash cells 5x with 1x PBS.
- Mount in Dapi.
Solutions (prepare fresh the same day of staining)
- * 1x Phosphate buffered saline.
- * Blocking reagent: 1% NCS in 1x PBS (use fresh 10x PBS).
- * Fixation solution: methanol:acetone 1: 1 ice cold.
Western Blotting Protocol
- Transfer gel to PDVF or nitrocellulose membrane
- Place membrane in plastic tray in blocking buffer for one hour with agitation
- Rinse in wash buffer
- Incubate in wash buffer plus primary antibody for one hour
- Wash 6 X 5 minutes with wash buffer
- Incubate in wash buffer plus secondary antibody for one hour
- Wash 6X 5 minutes with wash buffer
- Detect (e.g. ECL, Amersham according to manufacturers instructions)
Wash buffer
PBS + 0.1% Tween 20
Blocking buffer
Wash buffer + 5% dried milk powder
The concentration of antibodies used depends on each antibody, the amount of antigen and the detection method used. Generally, dilution is in the range of a few hundred times dilution to a few thousand times dilution, but usually has to be determined empirically.
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Price and other information provided are subject to change without notice. While every effort is made to keep information provided Up to date, ImmuQuest will not be liable if errors should occur in such information.
Formal acceptance of an order will take place when the goods are dispatched. If prices should be changed between the time of receipt of an order and dispatch, ImmuQuest will contact you in advance.
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We are strongly committed to providing the best quality products, and the best service possible.
Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.
If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.
Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.
Product Guarantee
We are strongly committed to providing the best quality products, and the best service possible. In order to do this we depend on your feedback.
Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.
If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.
Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.
If you have any questions please contact us using the following details:
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TS9 5JZ
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Tel: 01642 713533
Fax: 01642 713988
International Tel: +44 1642 713533
International Fax: +44 1642 713988
When placing an order ImmuQuest require a purchase order number, plus name and contact details of the purchaser, and the person who will be using the product. ImmuQuest will also need a VAT number for customers in the European Union.UK customers that are VAT exempt need to fax an exemption certificate.
Orders can be placed either by our website, via email or by mail. Please see our contact us page for more details. All orders are subject to availability. Prices of products do not include shipping, VAT or import duties where these are applicable.
Price and other information provided are subject to change without notice. While every effort is made to keep information provided Up to date, ImmuQuest will not be liable if errors should occur in such information. Formal acceptance of an order will take place when the goods are dispatched. If prices should be changed between the time of receipt of an order and dispatch, ImmuQuest will contact you in advance.
