Western blot showing Anti-Plakophilin 1 [19F10] on HaCat keratinocyte cells

Western blot showing Anti-Ajuba (21G4) on HaCat keratinocyte cells

Western blot showing SLC9A3R2 Antibody [32B6] on HaCat keratinocyte cells

Frozen section of Mouse kidney stained with Hamster Anti-Podoplanin/gp36 [clone 8.1.1]

Kallikreins (KLKs) are a subgroup of trypsin-like serine proteases and are implicated in carcinogenesis Kallikrein 2 is most closely related to Kallikrein 3 (PSA) with 78 identity and with kallikrein 1 at 68 identity Like Kallikrein 3 Kallikrein 2 is found in greatest abundance in the prostate but at much lower levels than Kallikrein 3 In addition Kallikrein 2 seems to be more labile than Kallikrein 1 or Kallikrein 3 and is often found in a cleaved form Kallikrein 2 is inhibited by Protein C Inhibitor (PCI)

Immunohistochemistry showing Anti-Cytokeratin K10-1 on formalin fixed paraffin embedded skin tissue

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - hair cortex Cytokeratin antibody [AE13] staining mouse skin tissue sections

Immunohistochemistry of Anti-Cytokeratin 13 [AE8] on formalin fixed paraffin embedded tonsil tissue

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 3 antibody [AE5] staining Cytokeratin 3 (red) in transfected Ptk2 cells

Human kidney (parafin embedded) stained with Cytokeratin type I (AE1)

MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators.hMAD2 is the human homologue of yeast MAD2

MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization

NEK2 is closely related in its catalytic domain to the serine/threonineprotein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers