Cell Signalling Antibodies

This range of Immuquest cell cycle/signalling/transcription antibodies supports a broad range of cell cycle, replication and protein transport mechanisms.

These cell cycle/signalling/transcription antibodies are implicated in a broad range of disease areas including cancer therapy.

Cell cycle /signalling/transcription factor antibodies are available in the following volumes including 0.05 ml, 0.1ml, 0.2 ml, 1 ml and 2 ml.

FANCD2 (pSer717) [P-CS-5]
Product Code:IQ655
Reactivity: Human
Application: WB, ELISA
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Western blot analysis of U87 cells either treated (RH) or untreated with temezolamide prior to analysis of total lysate either with indicated phospho-specific antibody or anti-FANCD2 antibody by immunoblotting blotting.

BRCA2 (pSer3291) [P-CS-4]
Product Code:IQ654
Reactivity: Human
Application: ELISA, WB, IB
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Western blot analysis of Hela cells either treated (LH) or untreated with nocodazole prior to analysis of total lysate either with indicated phospho-specific antibody or anti-BRCA2 antibody by immunoblotting blotting.

Upf1/Rent1 (pThr28) Antibody [P-CS-1]
Product Code:IQ653
Reactivity: Human
Application: WB, IB, ELISA
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Western blot analysis of wild-type and phospho-site mutant Flag-tagged Upf1 isogenically expressed in Hela cells and immuno-precipitated with anti-Flag antibody prior to blotting.

Desmoglein 2/DSG2 Monoclonal Antibody [10D2]
Product Code:IQ579
Reactivity: Human (Predicted to work with: Mouse)
Application: WB, ICC/IF, IP
Citation Count: 1
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Western blot - Anti-Desmoglein 2/DSG2 (10D2) on HaCat keratinocyte cells

NCAPD3/hCAP-D3 Monoclonal Antibody [2B5]
Product Code:IQ554
Reactivity: Human
Application: WB, IP
Citation Count: 2
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Western blot of HeLa cell lysate with Mouse anti NCAPD3/HCAP-D3 monoclonal antibody, clone 2B5. Antibody used at 2.5ug/ml, detection with anti-mouse Fc HRP conjugate, with 90 second ECL exposure. A protein band at approximately 170-180kD is seen, consistent with the expected MW of NCAPD3.

HDAC3 Monoclonal Antibody [3G6]
Product Code:IQ553
Reactivity: Human, Mouse
Application: WB , IP, ICC/IF
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NIH3T3 mouse fibroblast lysates were separated by 10% SDS-PAGE and blotted onto nitrocellulose membrane. The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C o/n with anti HDAC3, clone 3G6 antibody at the indicated dilutions. A 30sec exposure is shown.

HDAC1 Monoclonal Antibody [10E2]
Product Code:IQ555
Reactivity: Mouse, Rat, Human
Application: Flow Cyt, WB, ICC/IF, IP, ChIP, IHC-P
Citation Count: 1
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HeLa lysate run on 4-12% Bis-Tris 2D gel in 1 x MOPS running buffer. Transfer to 0.45µm nitrocellulose. Membrane probed with 10E2 (HDAC1). Anti-mouse IgG (whole molecule)-AP conjugate (1 in 2,000). Detection with BCIP/NBT substrate

FNIP1 Polyclonal Antibody
Product Code:IQ351
Reactivity: Mouse, Human
Application: WB, IP, IB
Citation Count: 1
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HEF1/NEDD-9 Monoclonal Antibody [14A11]
Product Code:IQ299
Reactivity: Human
Application: WB, IP, ICC/IF
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Aurora B Antibody
Product Code:IQ234
Reactivity: Human
Application: WB, IHC(P)
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IHC using Aurora B antibody (IQ234) on Human skin

Acetyl Lysine Monoclonal Antibody [7F8]
Product Code:IQ355
Reactivity: Species Independant
Application: IHC-P, IHC-Fr, WB, ELISA
Citation Count: 3
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ELISA -Anti Acetyl Lysine Antibody [7F8]

Emerin Monoclonal Antibody [MANEM1(5D10)]
Product Code:IQ567
Reactivity: Rabbit, Human.Does not react with Mouse
Application: ICC/IF, WB
Citation Count: 1
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IF - Emerin Monoclonal Antibody [MANEM1(5D10)] on normal Human fibroblasts

HDAC2 Monoclonal Antibody [3F3]
Product Code:IQ544
Reactivity: Mouse, Rat, Human. Predicted to work with Chicken, Cow
Application: Flow Cyt, IHC-P, WB, IP, ChIP, ICC/IF
Citation Count: 24
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HeLa lysate run on 4-12% Bis-Tris 2D gel in 1 x MOPS running buffer. Transfer to 0.45µm nitrocellulose. Membrane probed with 3F3 (anti-HDAC2). Anti-mouse IgG (whole molecule)-AP conjugate (1 in 2,000). Detection with BCIP/NBT substrate

HEF1/NEDD-9 Monoclonal Antibody [2G9]
Product Code:IQ296
Reactivity: Mouse, Rat, Human
Application: WB, IP, ICC/IF, IHC-Fr, Flow Cyt
Citation Count: 34
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Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Histone H4 (mono methyl K20) Monoclonal Antibody [5E10-D8]
Product Code:IQ551
Reactivity: Human. Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals
Application: Dot blot, WB against endogenous protein, ELISA, ChIP, IHC-P, ICC/IF, Flow Cyt
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Histone H4 (tri methyl K20) Monoclonal Antibody [6F8-D9]
Product Code:IQ558
Reactivity: Human, Caenorhabditis elegans. Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals
Application: Dot blot, WB against endogenous protein, ELISA, ChIP
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(1µg/ml) staining in NIH3T3 cells nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LAP2 alpha Monoclonal Antibody [3A3]
Product Code:IQ559
Reactivity: Human
Application: WB, IP, ICC/IF
Citation Count: 2
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Hela cells were boiled in Laemmli buffer and the proteins were separated by 8% SDS-PAGE and blotted onto nitrocellulose membrane. The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C o/n with anti LAP2α, clone 3A3 antibody at the indicated dilution in 0.5% NFDM/PBS-T. After incubation with anti-mouse Fc-HRP coupled secondary antibody for 1h at RT, ECL was performed with the Western Lightning reagents. A 30sec exposure is shown.

Nidogen/Enactin Monoclonal Antibody [JF2]
Product Code:IQ314
Reactivity: Mouse, Human
Application: WB, ELISA, Blocking
Citation Count: 1
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Nidogen/Enactin Monoclonal Antibody [JF2]