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Current Image Legend: Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.




Transportin 1/MIP Monoclonal Antibody [D45]

Catalogue Number: IQ223

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Download Variant Datasheet
Primary Description: Mouse Anti-Transportin 1 [D45]
Target Antigen: Transportin 1
Catalogue Number: IQ223
Quantity: 0.1mg
Concentration: 1mg/ml
Clone: D45
Host: Mouse
Isotype: IgG1
Myeloma/Fusion Partners: SP2/0
Immunogen: Recombinant his tagged transportin 1 (Human)
Species Reactivity: Mouse, Rabbit, Human, Monkey
Purification: Protein A
Format: Purified antibody (from supernatant) containing PBS 0.1% sodium azide
Applications: Flow Cyt, ICC/IF, IHC-P, ELISA, WB, IP
Dilutions: Optimal antibody dilution should be determined by titration, however as a guideline try;Flow Cyt: Use 1µg for 106 cells.ICC/IF: Use a concentration of 5 µg/mlIHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.WB: 1:8000. Detects a band of approximately 93 kDa (predicted molecular weight: 107 kDa)
Also Known As: Importin beta 2 antibody;IPO 2 antibody;IPO2 antibody;Karyopherin (importin) beta 2antibody;Karyopherin beta 2 antibody;KPN B2 antibody;KPNB 2 antibody;KPNB2 antibody;M9 interacting proteinantibody;M9 region interacting protein antibody;M9 region interaction protein antibody;M9-interacting proteinantibody;MIP 1 antibody;MIP antibody;MIP1 antibody;TNPO 1 antibody;TNPO1 antibody;Transportinantibody;Transportin-1 antibody;Transportin1 antibody;TRN antibody
Pubmed ID(s): 22411826, 21053360, 21401918, 21847626, 20421341, 20663864, 18846510, 9298975
Entrez Gene ID(s): 3842, 238799
SwissProt ID(s): Q92973, Q8BFY9