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  • ICC/IF using IQ212 (4D11) on paraformaldehyde fixed HeLa cells,  permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.
  • WB using IQ212 (4D11) (0.001µg/ml) staining in Jurkat(A) and HepG2(B) cells nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - hnRNP L antibody [4D11] on Human normal skin

Current Image Legend:




hnRNP L Monoclonal Antibody [4D11]

Catalogue Number: IQ212

Catalogue Number: IQ268

Catalogue Number: IQ212AF

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  • £328.90

Datasheet

Download Variant Datasheet
Primary Description: Mouse Anti-hnRNP L [4D11]
Primary Description: Mouse Anti-hnRNP L [4D11]
Primary Description: hnRNP L Antibody [4D11]- BSA/Azide Free
Target Antigen: hnRNP L
Target Antigen: hnRNP L
Target Antigen: hnRNP L
Catalogue Number: IQ212
Catalogue Number: IQ268
Catalogue Number: IQ212AF
Quantity: 0.1mg
Quantity: 0.05mg
Quantity: 0.05mg
Concentration: 1mg/ml
Concentration: 1mg/ml
Concentration: 1mg/ml
Clone: 4D11
Clone: 4D11
Clone: 4D11
Host: Mouse
Host: Mouse
Host: Mouse
Isotype: IgG1
Isotype: IgG1
Isotype: IgG1
Myeloma/Fusion Partners: SP2/0
Myeloma/Fusion Partners: SP2/0
Myeloma/Fusion Partners: Sp2/0
Immunogen: Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose
Immunogen: Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose
Immunogen: Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose
Species Reactivity: Mouse, Rat, Human, Newt
Species Reactivity: Mouse, Rat, Human, Newt
Purification: Protein A
Purification: Protein A
Purification: Protein A
Format: Purified antibody (from supernatant) containing PBS 0.1% sodium azide;
Format: Purified antibody (from supernatant) containing PBS 0.1% sodium azide
Format: Purified antibody (from supernatant) containing PBS. Does not contain sodium azide or BSA
Applications: IHC-Fr, IHC-P, ELISA, WB, IP, Flow Cyt, ICC/IF
Applications: IHC-Fr, IHC-P, ELISA, WB, IP, Flow Cyt, ICC/IF
Applications: IHC-Fr, IHC-P, ELISA, WB, IP, Flow Cyt, ICC/IF
Dilutions: Optimal antibody dilution should be determined by titration, however as a guideline try; IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ICC/IF: 1:100 on paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. WB: (0.001µg/ml) staining in Jurkat and HepG2 cells nuclear lysate. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).Flow Cyt: Use 1µg for 106 cells.
Dilutions: Optimal antibody dilution should be determined by titration, however as a guideline try; IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB: 1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Flow Cyt: Use 1µg for 106 cells
Dilutions: Optimal antibody dilution should be determined by titration, however as a guideline try; IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB: 1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Flow Cyt: Use 1µg for 106 cells.
Also Known As: D830027H13Rik antibody;FLJ35509 antibody;Heterogeneous nuclear ribonucleoprotein L antibody;hnRNPLantibody;hnRPL antibody;P/OKcl.14 antibody
Pubmed ID(s): 29293066, 30237493, 27658966, 28038443, 28262838, 28088793, 28881634, 28512194, 26704980, 27317697, 26110767, 26468954, 26443849, 24371310, 24285722, 24434558, 24164894, 24671418, 23161687, 21507955, 20932480, 19244236, 18945760, 19689476, 16980303, 17095106, 16001081, 18644864, 17664280, 2687284
Pubmed ID(s): 29293066, 30237493, 27658966, 28038443, 28262838, 28088793, 28881634, 28512194, 26704980, 27317697, 26110767, 26468954, 26443849, 24371310, 24285722, 24434558, 24164894, 24671418, 23161687, 21507955, 20932480, 19244236, 18945760, 19689476, 16980303, 17095106, 16001081, 18644864, 17664280, 2687284
Pubmed ID(s): 29293066, 30237493, 27658966, 28038443, 28262838, 28088793, 28881634, 28512194, 26704980, 27317697, 26110767, 26468954, 26443849, 24371310, 24285722, 24434558, 24164894, 24671418, 23161687, 21507955, 20932480, 19244236, 18945760, 19689476, 16980303, 17095106, 16001081, 18644864, 17664280, 2687284
Entrez Gene ID(s): 3191, 15388, 80846
Entrez Gene ID(s): 3191, 15388, 80846
Entrez Gene ID(s): 3191, 15388, 80846
SwissProt ID(s): p14866, q8r081, P31943
SwissProt ID(s): P14866, Q8R081, P31943
SwissProt ID(s): p14866, q8r081, P31943
Omim ID(s): 603083
Omim ID(s): 603083

Citations

Citation Count: 30
Citations:

Majewski L et al. Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. Nucleus 9:125-141 (2018).
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Chen C et al. LNMAT1 promotes lymphatic metastasis of bladder cancer via CCL2 dependent macrophage recruitment. Nat Commun 9:3826 (2018).
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Gazzara MR et al. Ancient antagonism between CELF and RBFOX families tunes mRNA splicing outcomes. Genome Res 27:1360-1370 (2017).
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Orecchini E et al. ADAR1 restricts LINE-1 retrotransposition. Nucleic Acids Res 45:155-168 (2017).
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Zhou X et al. HnRNP-L promotes prostate cancer progression by enhancing cell cycling and inhibiting apoptosis. Oncotarget 8:19342-19353 (2017). IP ; Human .
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Coppin L et al. Galectin-3 is a non-classic RNA binding protein that stabilizes the mucin MUC4 mRNA in the cytoplasm of cancer cells. Sci Rep 7:43927 (2017).
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Lv D et al. HnRNP-L mediates bladder cancer progression by inhibiting apoptotic signaling and enhancing MAPK signaling pathways. Oncotarget 8:13586-13599 (2017).
View Source


Seo JY et al. Heterogeneous nuclear ribonucleoprotein (hnRNP) L promotes DNA damage-induced cell apoptosis by enhancing the translation of p53. Oncotarget 8:51108-51122 (2017). WB ; Mouse .
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Ajiro M et al. A genome landscape of SRSF3-regulated splicing events and gene expression in human osteosarcoma U2OS cells. Nucleic Acids Res 44:1854-70 (2016).
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Llorian M et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res 44:8933-8950 (2016). WB ; Mouse .
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Martinez NM et al. Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activation. Genes Dev 29:2054-66 (2015).
View Source


Kim SY et al. Acetylome Analysis Identifies SIRT1 Targets in mRNA-Processing and Chromatin-Remodeling in Mouse Liver. PLoS One 10:e0140619 (2015).
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PreviousRoda D et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS One 10:e0130543 (2015). Human .
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Motta-Mena LB et al. An optogenetic gene expression system with rapid activation and deactivation kinetics. Nat Chem Biol 10:196-202 (2014).
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Michel M et al. Activation-induced tumor necrosis factor receptor-associated factor 3 (Traf3) alternative splicing controls the noncanonical nuclear factor ?B pathway and chemokine expression in human T cells. J Biol Chem 289:13651-60 (2014).
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Shankarling G et al. Transcriptome-wide RNA interaction profiling reveals physical and functional targets of hnRNP L in human T cells. Mol Cell Biol 34:71-83 (2014).
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Giraud M et al. An RNAi screen for Aire cofactors reveals a role for Hnrnpl in polymerase release and Aire-activated ectopic transcription. Proc Natl Acad Sci U S A 111:1491-6 (2014).
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Gregory BL & Cheung VG Natural variation in the histone demethylase, KDM4C, influences expression levels of specific genes including those that affect cell growth. Genome Res 24:52-63 (2014).
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Li Z et al. The long noncoding RNA THRIL regulates TNFa expression through its interaction with hnRNPL. Proc Natl Acad Sci U S A 111:1002-7 (2014). WB ; Human .
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Peddigari S  et al. hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition. Nucleic Acids Res 41:575-85 (2013). WB ; Human
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Peddigari S et al. hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition. Nucleic Acids Res 41:575-85 (2013). WB ; Human .PubMed: 23161687ive splicing of neurexin 2. J Mol Neurosci 50:221-33 (2013). WB .
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Gazzara MR et al. In silico to in vivo splicing analysis using splicing code models. Methods N/A:N/A (2013).
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Motta-Mena LB  et al. A Disease-associated Polymorphism Alters Splicing of the Human CD45 Phosphatase Gene by Disrupting Combinatorial Repression by Heterogeneous Nuclear Ribonucleoproteins (hnRNPs). J Biol Chem 286:20043-53 (2011). WB ; Human
View Source


Motta-Mena LB et al. A Disease-associated Polymorphism Alters Splicing of the Human CD45 Phosphatase Gene by Disrupting Combinatorial Repression by Heterogeneous Nuclear Ribonucleoproteins (hnRNPs). J Biol Chem 286:20043-53 (2011). WB ; Human .
View Source


Heyd F & Lynch KW Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing. Mol Cell 40:126-37 (2010). WB ; Human
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Heyd F & Lynch KW Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing. Mol Cell 40:126-37 (2010). WB ; Human .
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Niu Y  et al. Identification of peptides applicable as vaccines for HLA-A26-positive cancer patients. Cancer Sci 100:2167-74 (2009). Flow Cyt ; Human
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Jia R  et al. Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol 83:167-80 (2009). WB ; Human
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Wang E & Cambi F Heterogeneous Nuclear Ribonucleoproteins H and F Regulate the Proteolipid Protein/DM20 Ratio by Recruiting U1 Small Nuclear Ribonucleoprotein through a Complex Array of G Runs. J Biol Chem 284:11194-204 (2009). WB
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Wang E & Cambi F Heterogeneous Nuclear Ribonucleoproteins H and F Regulate the Proteolipid Protein/DM20 Ratio by Recruiting U1 Small Nuclear Ribonucleoprotein through a Complex Array of G Runs. J Biol Chem 284:11194-204 (2009). WB .
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Jia R et al. Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol 83:167-80 (2009). WB ; Human .
View Source


Niu Y et al. Identification of peptides applicable as vaccines for HLA-A26-positive cancer patients. Cancer Sci 100:2167-74 (2009). Flow Cyt ; Human .
View Source


Venables JP  et al. Multiple and specific mRNA processing targets for the major human hnRNP proteins. Mol Cell Biol 28:6033-43 (2008). WB ; Human
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Venables JP et al. Multiple and specific mRNA processing targets for the major human hnRNP proteins. Mol Cell Biol 28:6033-43 (2008). WB ; Human .
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Melton AA  et al. Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol 27:6972-84 (2007). WB ; Human
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Melton AA et al. Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol 27:6972-84 (2007). WB ; Human .
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Griffith BN et al. Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing. Biochim Biophys Acta. 2006 Nov-Dec;1759(11-12):552-61. Epub 2006 Oct 6. WB ; Mouse 
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Szeszel-Fedorowicz W et al. An exonic splicing silencer is involved in the regulated splicing of glucose 6-phosphate dehydrogenase mRNA. J Biol Chem. 2006 Nov 10;281(45):34146-58. Epub 2006 Sep 15. WB ; Human, Rat
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Rothrock CR  et al. HnRNP L represses exon splicing via a regulated exonic splicing silencer. EMBO J 24:2792-802 (2005)
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Rothrock CR et al. HnRNP L represses exon splicing via a regulated exonic splicing silencer. EMBO J 24:2792-802 (2005).
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A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts. Pinol Roma S, Swanson MS, Gall JG, Dreyfuss G ( The Journal of Cell Biology,1989)
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Protocols

Immunofluorescence protocol - Formaldehyde fixation
  1. Collect cells from T.c.unit and remove media from petri dish using suction.
  2. Wash with 1x PBS and remove.
  3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperature on an orbital shaker.
  4. Remove PFA and incubate in 0.5% Triton X-IOO in 1x PBS for 5 minutes at room temperature.
  5. Prepare blocking reagent, this is also the antibody diluent.
  6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker.
  7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature.
  8. Prepare primary antibodies (50?l/coverslip) and moist staining chambers.
  9. Wash cells 2x with lx PBS at room temperature and air dry briefly.
  10. Incubate with primary antibody for 1 hr at room temperature in the dark in staining chambers. During this time prepare the secondary antibody.
  11. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)
  12. Incubate with secondary antibody for 1 hour at room temperature in the dark in staining chambers.
  13. Wash cells 5x with 1x PBS.
  14. Mount in Dapi.

Solutions (prepare fresh the same day of staining).

  • * 1x Phosphate buffered saline.
  • * Blocking reagent: 1% NCS in 1x PBS (use fresh l0x PBS).
  • * Fixation solution: 3.5% Para formaldehyde.

1.75g PFA in 20 ml d.H20 plus 5 drops 1M NaOH. Stir on a hot plate at 50-60°C until dissolved. Add 4 drops IN HCI and check pH indicator strip. PH should be 7.4. Complete volume with d.H20 to 25ml and add 25ml 2xPBS. Check pH before adding to cover slips.

Immunofluorescence protocol - Methanol/acetone fixation
  1. Collect cells from T.C.unit and remove media from petri dish using suction.
  2. Wash with 1x PBS and remove.
  3. Fix cells with cold methanol: acetone 1: 1 for 10 minutes on ice.
  4. Prepare blocking reagent, this is also the diluent for the antibodies.
  5. Remove fixative and wash cells 3x with Ix PBS at RT, for 4 minutes/wash on orbital shaker.
  6. Block with 1% NCS and Ix PBS for 30 minutes at RT.
  7. Prepare primary antibodies (50?l/coverslip) and moist staining chambers.
  8. Wash cells 2x with 1 x PBS at RT and air dry for approximately 7 minutes.
  9. Incubate with primary antibody for 1 hr at RT in the dark in staining chambers. During this time prepare secondary antibody.
  10. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)
  11. Incubate with secondary antibody for 1 hr at R T in the dark in staining chambers.
  12. Wash cells 5x with 1x PBS.
  13. Mount in Dapi.

Solutions (prepare fresh the same day of staining)

  • * 1x Phosphate buffered saline.
  • * Blocking reagent: 1% NCS in 1x PBS (use fresh 10x PBS).
  • * Fixation solution: methanol:acetone 1: 1 ice cold.
Western Blotting Protocol
  1. Transfer gel to PDVF or nitrocellulose membrane
  2. Place membrane in plastic tray in blocking buffer for one hour with agitation
  3. Rinse in wash buffer
  4. Incubate in wash buffer plus primary antibody for one hour
  5. Wash 6 X 5 minutes with wash buffer
  6. Incubate in wash buffer plus secondary antibody for one hour
  7. Wash 6X 5 minutes with wash buffer
  8. Detect (e.g. ECL, Amersham according to manufacturers instructions)
Wash buffer

PBS + 0.1% Tween 20

Blocking buffer

Wash buffer + 5% dried milk powder

The concentration of antibodies used depends on each antibody, the amount of antigen and the detection method used. Generally, dilution is in the range of a few hundred times dilution to a few thousand times dilution, but usually has to be determined empirically.

FAQ's

How do I place an order?

When placing an order we require a purchase order number, plus name and contact details of the purchaser, and the person who will be using the product. We will add VAT for every order received from an EU country, unless you are VAT exempt. For such VAT exempt customers we will need to receive a copy of the exemption certificate with the order.

Orders can be placed either by our website, via email or by mail. Please see our contact us page for more details

All orders are subject to availability. Prices of products do not include shipping, VAT or import duties, where these are applicable. Price and other information provided are subject to change without notice. While every effort is made to keep information provided Up to date, ImmuQuest will not be liable if errors should occur in such information.

Formal acceptance of an order will take place when the goods are dispatched. If prices should be changed between the time of receipt of an order and dispatch, ImmuQuest will contact you in advance.

Ordering via the website

To order via the website you will need to find the product(s) you are looking for by either using our search, browsing our categories or using our full antibody catalogue.

Once you have found the antibodies you are looking for you can view more detailed information about a specific antibody by clicking the product or the 'View' button. Once you are sure it is the antibody you are looking for you can add it to your order by clicking the 'Add To Order' button.

This places the antibody in your order basket, you can add as many antibodies as you wish. Once you have selected all of the antibodies you wish to order then click the checkout button. If you are not logged in you will be asked to login or to register, if you are logged in you will be taken to the checkout page.

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Delivery usually takes no more than one week from reciept of an order.

Do you guarantee your antibodies?

Yes. Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

Do you charge VAT?

If you are in the EU and you are no exempt from paying VAT then we must charge VAT (charged at the prevailing UK rate).

Customers that are exempt need to fax an exemption form showing their vat exemption number.

What if I don't have a purchase order number?

You can still place an order without providing a purchase order number but we must recieve payment before your order is despatched.

Non-website orders

Non-website orders can be placed directly with Immuquest at sales@immuquest.com or click on the "contact us" tab for address details

Delivery

Offline Orders

For offline orders please call +44 (0) 1642 713533 or click here to email us.

Delivery & returns

We use federal Express for UK & international shipments, andfor standard shipments the cost is £30 UK & £55 international. If the customer uses their account, we charge a £20 handling fee (UK & international)

We are strongly committed to providing the best quality products, and the best service possible.

Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

Product Guarantee

We are strongly committed to providing the best quality products, and the best service possible. In order to do this we depend on your feedback.

Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

If you have any questions please contact us using the following details:

ImmuQuest Ltd
Springboard
Stokesley Business Park
24 Ellerbeck Way
Stokesley
TS9 5JZ
UK

Tel: 01642 713533
Fax: 01642 713988
International Tel: +44 1642 713533
International Fax: +44 1642 713988

When placing an order ImmuQuest require a purchase order number, plus name and contact details of the purchaser, and the person who will be using the product. ImmuQuest will also need a VAT number for customers in the European Union.UK customers that are VAT exempt need to fax an exemption certificate.

Orders can be placed either by our website, via email or by mail. Please see our contact us page for more details. All orders are subject to availability. Prices of products do not include shipping, VAT or import duties where these are applicable.

Price and other information provided are subject to change without notice. While every effort is made to keep information provided Up to date, ImmuQuest will not be liable if errors should occur in such information. Formal acceptance of an order will take place when the goods are dispatched. If prices should be changed between the time of receipt of an order and dispatch, ImmuQuest will contact you in advance.