Introducing a unique monoclonal antibody recognizing both wild Cas9 and inactive Cas (dCas)
Clustered regularly interspaced short palindromic repeat (CRISPR) and associated CRISP-R associated nuclease (Cas) proteins comprise the CRISPR/Cas systems responsible for adaptive immunity in many bacteria and archaea. Three types of CRISPR/Cas systems have been identified to date, designated types I, II and III.
Cas9 is an RNA guided DNA nuclease associated with the type II CRISPR/Cas adaptive immune systems.
The CRISPR/Cas9 system protects the viruses and plasmids in prokaryotes by targeting foreign nucleic acids and with complementary single-stranded RNA. This localizes the Cas9 nuclease to the target DNA sequence, introducing double-strand breaks at specific locations with the genomic DNA.
In recent years, scientists have recognized the importance of the CRISPR/Cas system as a tool for genomic editing and engineering. In particular, the type II CRISP/Cas9 derived from Streptococcus pyogenes has been adapted and utilized as the genome engineering tool of choice.
The CRISPR/Cas9 system has the potential to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism.
In response to the growing interest in CRISPR/Cas9, Immuquest offers a unique monoclonal antibody (clone 7A9-3A3) against Cas9 nuclease (product code IQ651). The antibody 7A9-3A3 is one of the first monoclonal antibodies against Cas9 on the market. The antibody was raised against an amino acid sequence from Streptococcus pyogenes cas9 and detects wild-type Cas9 and the catalytically inactive dCas9. The antibody is suitable for detecting Cas9 by various techniques, including immunofluorescence, western blotting and immunoprecipitation. IQ651 is an invaluable tool for identifying CRISPR/Cas9 in experimental systems.
- Monoclonal antibody raised against Streptococcus pyogenes Cas9 (clone 7A9-3A3)
- IQ651 recognizes both wild type Cas9 and Cas9 (dCas9)
- IQ651 is suitable for use in a wide range of applications
- Western blotting