Sale
  • Gemin 1 Antibody [2B1]

Current Image Legend:




Gemin 1 Antibody [2B1]

Catagloue Number: IQ259

Item in Stock Secure Online Payments

If you have any questions or require further information, please contact us and one of our scientists will be in touch.

A free sample may be available. Please click here to request this.

  • £202.00

Datasheet

Target Antigen: Gemin 1
Catalogue Number: IQ259
Quantity: 50ul 
Concentration: 1.8mg/ml
Clone: 2B1
Host: Mouse
Myeloma/Fusion Partners: A/J Sp2/0 cell line
Immunogen: Purified recombinant His6 tagged-SMN protein (human)
Species Reactivity: Mouse, Human, Xenopus laevis
Purification: Protein A
Format: Purified antibody (from supernatant) containing PBS + 0.1% sodium az
Applications: IHC-P, ICC/IF, ELISA, WB, IP, Flow Cyt
Dilutions: Optimal antibody dilution should be determined by titration, however as a guideline try IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocolWB: Use at an assay dependent dilution. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa)Flow Cyt: Use 1µg for 106 cells
Also Known As: BCD541 antibody;Gemin 1 antibody;Gemin-1 antibody;Gemin1 antibody;OTTHUMP00000125198antibody;OTTHUMP00000223567 antibody;OTTHUMP00000223568 antibody;OTTHUMP00000224066antibody;OTTHUMP00000226924 antibody;SMA 1 antibody;SMA 2 antibody;SMA 3 antibody;SMA 4 antibody;SMAantibody;SMA@ antibody;SMA1 antibody;SMA2 antibody;SMA3 antibody;SMA4 antibody;SMN 1 antibody;SMNantibody;SMN-1 antibody;SMN1 antibody;SMNT antibody;Survival motor neuron protein antibody;Survival ofmotor neuron 1 (telomeric) antibody;survival of motor neuron 1 antibody;Survival of motor neuron 1, telomericantibody;T-BCD541 antibody
Pubmed ID(s): 31444317, 28232485, 27352031, 25943887, 24866055, 24792162, 24978456, 25263560, 25169827, 24155930, 24038356, 22641158, 21285413, 19506027, 17371836, 30592264, 30896889, 31442237, 31799068, 30450775, 29537490, 30032146, 30247708, 30592139, 29201199, 25535084, 25628358, 23740936
Entrez Gene ID(s): 3185, 3187, 6606, 6607, 20595
SwissProt ID(s): p31943, p52597, q16637, p97801
Unigene ID(s): 604001, 808, 202179, 535788, 2025
Omim ID(s): 601035, 601037, 600354
Citation Count: 28
Citations:

Jiang D et al. Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells. FEBS Lett 592:1400-1411 (2018).
View Source


Jiang ZJ et al. Galectin-1 Restores Immune Tolerance to Liver Transplantation Through Activation of Hepatic Stellate Cells. Cell Physiol Biochem 48:863-879 (2018).
View Source


Ma L et al. MicroRNA-219 overexpression serves a protective role during liver fibrosis by targeting tumor growth factor ß receptor 2. Mol Med Rep 19:1543-1550 (2019).
View Source


Zhang H et al. Overexpression of c-Ski promotes cell proliferation, invasion and migration of gastric cancer associated fibroblasts. Kaohsiung J Med Sci 35:214-221 (2019).
View Source


Zhang Y et al. Silencing Nogo-B receptor inhibits penile corpus cavernosum vascular smooth muscle cell apoptosis of rats with diabetic erectile dysfunction by down-regulating ICAM-1. PLoS One 14:e0220715 (2019).
View Source


Che X et al. Long noncoding RNA MEG3 suppresses podocyte injury in diabetic nephropathy by inactivating Wnt/ß-catenin signaling. PeerJ 7:e8016 (2019).
View Source


Wei XF et al. Wnt and BMP signaling pathways co-operatively induce the differentiation of multiple myeloma mesenchymal stem cells into osteoblasts by upregulating EMX2. J Cell Biochem N/A:N/A (2018).
View Source


Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018).
View Source


Li Q et al. Chemically modified liposomes carrying TRAIL target activated hepatic stellate cells and ameliorate hepatic fibrosis in vitro and in vivo. J Cell Mol Med N/A:N/A (2018).
View Source


Chen Z et al. LYTAK1 attenuates proliferation of retinal pigment epithelial cells through TGF-ß-mediated epithelial-mesenchymal transition via the ERK/AKT signaling pathway. Exp Ther Med 14:4951-4957 (2017).
View Source


Chen T et al. MicroRNA-199b Modulates Vascular Cell Fate During iPS Cell Differentiation by Targeting the Notch Ligand Jagged1 and Enhancing VEGF Signaling. Stem Cells 33:1405-18 (2015).
View Source


Schertzer M et al. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA. Nucleic Acids Res 43:1834-47 (2015). Human .
View Source


Ishihara T et al. Decreased number of Gemini of coiled bodies and U12 snRNA level in amyotrophic lateral sclerosis. Hum Mol Genet 22:4136-47 (2013).
View Source


Li M et al. LncRNA RP11-670E13.6, interacted with hnRNPH, delays cellular senescence by sponging microRNA-663a in UVB damaged dermal fibroblasts. Aging (Albany NY) 11:5992-6013 (2019).
View Source


Li W et al. Long non-coding RNA LINC00672 contributes to p53 protein-mediated gene suppression and promotes endometrial cancer chemosensitivity. J Biol Chem 292:5801-5813 (2017).
View Source


Li R et al. Misregulation of Alternative Splicing in a Mouse Model of Rett Syndrome. PLoS Genet 12:e1006129 (2016).
View Source


Cooper-Knock J et al. Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. Acta Neuropathol 130:63-75 (2015). IHC .
View Source


Cooper-Knock J et al. Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions. Brain 137:2040-51 (2014). IHC-P ; Human .
View Source


Mandler MD et al. A cytoplasmic quaking I isoform regulates the hnRNP F/H-dependent alternative splicing pathway in myelinating glia. Nucleic Acids Res 42:7319-29 (2014). WB ; Mouse .
View Source


Reznik B et al. hnRNP F Complexes with Tristetraprolin and Stimulates ARE-mRNA Decay. PLoS One 9:e100992 (2014). IP ; Mouse .
View Source


Smith LD et al. A targeted oligonucleotide enhancer of SMN2 exon 7 splicing forms competing quadruplex and protein complexes in functional conditions. Cell Rep 9:193-205 (2014).
View Source


Widera M et al. A functional conserved intronic G run in HIV-1 intron 3 is critical to counteract APOBEC3G-mediated host restriction. Retrovirology 11:72 (2014). WB .
View Source


Smith LD et al. Intron Retention in the Alternatively Spliced Region of RON Results from Weak 3' Splice Site Recognition. PLoS One 8:e77208 (2013). IP .
View Source


Schmid R et al. The splicing landscape is globally reprogrammed during male meiosis. Nucleic Acids Res 41:10170-84 (2013). WB ; Mouse .
View Source


Marzano V et al. Proteomic profiling of ATM kinase proficient and deficient cell lines upon blockage of proteasome activity. J Proteomics 75:4632-46 (2012). WB ; Human .
View Source


Tsai BP et al. Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Mol Cell Proteomics 10:M110.007385 (2011). WB .
View Source


Millevoi S et al. A physical and functional link between splicing factors promotes pre-mRNA 3' end processing. Nucleic Acids Res 37:4672-83 (2009). IP .
View Source


Paronetto MP et al. The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x. J Cell Biol 176:929-39 (2007).
View Source


Protocols

Immunofluorescence protocol - Formaldehyde fixation
  1. Collect cells from T.c.unit and remove media from petri dish using suction.
  2. Wash with 1x PBS and remove.
  3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperature on an orbital shaker.
  4. Remove PFA and incubate in 0.5% Triton X-IOO in 1x PBS for 5 minutes at room temperature.
  5. Prepare blocking reagent, this is also the antibody diluent.
  6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker.
  7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature.
  8. Prepare primary antibodies (50?l/coverslip) and moist staining chambers.
  9. Wash cells 2x with lx PBS at room temperature and air dry briefly.
  10. Incubate with primary antibody for 1 hr at room temperature in the dark in staining chambers. During this time prepare the secondary antibody.
  11. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)
  12. Incubate with secondary antibody for 1 hour at room temperature in the dark in staining chambers.
  13. Wash cells 5x with 1x PBS.
  14. Mount in Dapi.

Solutions (prepare fresh the same day of staining).

  • * 1x Phosphate buffered saline.
  • * Blocking reagent: 1% NCS in 1x PBS (use fresh l0x PBS).
  • * Fixation solution: 3.5% Para formaldehyde.

1.75g PFA in 20 ml d.H20 plus 5 drops 1M NaOH. Stir on a hot plate at 50-60°C until dissolved. Add 4 drops IN HCI and check pH indicator strip. PH should be 7.4. Complete volume with d.H20 to 25ml and add 25ml 2xPBS. Check pH before adding to cover slips.

Immunofluorescence protocol - Methanol/acetone fixation
  1. Collect cells from T.C.unit and remove media from petri dish using suction.
  2. Wash with 1x PBS and remove.
  3. Fix cells with cold methanol: acetone 1: 1 for 10 minutes on ice.
  4. Prepare blocking reagent, this is also the diluent for the antibodies.
  5. Remove fixative and wash cells 3x with Ix PBS at RT, for 4 minutes/wash on orbital shaker.
  6. Block with 1% NCS and Ix PBS for 30 minutes at RT.
  7. Prepare primary antibodies (50?l/coverslip) and moist staining chambers.
  8. Wash cells 2x with 1 x PBS at RT and air dry for approximately 7 minutes.
  9. Incubate with primary antibody for 1 hr at RT in the dark in staining chambers. During this time prepare secondary antibody.
  10. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)
  11. Incubate with secondary antibody for 1 hr at R T in the dark in staining chambers.
  12. Wash cells 5x with 1x PBS.
  13. Mount in Dapi.

Solutions (prepare fresh the same day of staining)

  • * 1x Phosphate buffered saline.
  • * Blocking reagent: 1% NCS in 1x PBS (use fresh 10x PBS).
  • * Fixation solution: methanol:acetone 1: 1 ice cold.
Western Blotting Protocol
  1. Transfer gel to PDVF or nitrocellulose membrane
  2. Place membrane in plastic tray in blocking buffer for one hour with agitation
  3. Rinse in wash buffer
  4. Incubate in wash buffer plus primary antibody for one hour
  5. Wash 6 X 5 minutes with wash buffer
  6. Incubate in wash buffer plus secondary antibody for one hour
  7. Wash 6X 5 minutes with wash buffer
  8. Detect (e.g. ECL, Amersham according to manufacturers instructions)
Wash buffer

PBS + 0.1% Tween 20

Blocking buffer

Wash buffer + 5% dried milk powder

The concentration of antibodies used depends on each antibody, the amount of antigen and the detection method used. Generally, dilution is in the range of a few hundred times dilution to a few thousand times dilution, but usually has to be determined empirically.

FAQ's

How do I place an order?

When placing an order we require a purchase order number, plus name and contact details of the purchaser, and the person who will be using the product. We will add VAT for every order received from an EU country, unless you are VAT exempt. For such VAT exempt customers we will need to receive a copy of the exemption certificate with the order.

Orders can be placed either by our website, via email or by mail. Please see our contact us page for more details

All orders are subject to availability. Prices of products do not include shipping, VAT or import duties, where these are applicable. Price and other information provided are subject to change without notice. While every effort is made to keep information provided Up to date, ImmuQuest will not be liable if errors should occur in such information.

Formal acceptance of an order will take place when the goods are dispatched. If prices should be changed between the time of receipt of an order and dispatch, ImmuQuest will contact you in advance.

Ordering via the website

To order via the website you will need to find the product(s) you are looking for by either using our search, browsing our categories or using our full antibody catalogue.

Once you have found the antibodies you are looking for you can view more detailed information about a specific antibody by clicking the product or the 'View' button. Once you are sure it is the antibody you are looking for you can add it to your order by clicking the 'Add To Order' button.

This places the antibody in your order basket, you can add as many antibodies as you wish. Once you have selected all of the antibodies you wish to order then click the checkout button. If you are not logged in you will be asked to login or to register, if you are logged in you will be taken to the checkout page.

On the checkout page you are able to double check your order and enter a purchase order number if you have one.

Once you are happy your order is correct you can purchase the order with debit/credit card details and your order will be submitted. Once we have received and checked your order we will contact you to arrange payment and delivery.

How long does delivery take?

Delivery usually takes no more than one week from reciept of an order.

Do you guarantee your antibodies?

Yes. Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

Do you charge VAT?

If you are in the EU and you are no exempt from paying VAT then we must charge VAT (charged at the prevailing UK rate).

Customers that are exempt need to fax an exemption form showing their vat exemption number.

What if I don't have a purchase order number?

You can still place an order without providing a purchase order number but we must recieve payment before your order is despatched.

Non-website orders

Non-website orders can be placed directly with Immuquest at sales@immuquest.com or click on the "contact us" tab for address details

Delivery

Offline Orders

For offline orders please call +44 (0) 1642 713533 or click here to email us.

Delivery & returns

We use federal Express for UK & international shipments, andfor standard shipments the cost is £30 UK & £55 international. If the customer uses their account, we charge a £20 handling fee (UK & international)

We are strongly committed to providing the best quality products, and the best service possible.

Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

Product Garantee

We are strongly committed to providing the best quality products, and the best service possible. In order to do this we depend on your feedback.

Should any product not perform as described in the product literature, it will be replaced or a full refund will be given. The customer must notify ImmuQuest within 30 days after the goods have been received to request a replacement, forwarding complete test data as requested by ImmuQuest.

If items are ordered incorrectly by the customer, ImmuQuest will consider taking them back as long as they have been stored correctly and have not been opened or tampered with. Such orders may be subject to a 15% restocking charge on the items plus any shipping costs.

Requests for returns must have prior authorization from ImmuQuest, and must be made within 7 days of receipt of the items. Items must be returned in the same or equivalent packaging as originally dispatched, and by an equivalent method of delivery.

If you have any questions please contact us using the following details:

ImmuQuest Ltd
26 The Green
Seamer
North Yorkshire
TS9 5LP
UK

Tel: 01642 713533
Fax: 01642 713988
International Tel: +44 1642 713533
International Fax: +44 1642 713988

When placing an order ImmuQuest require a purchase order number, plus name and contact details of the purchaser, and the person who will be using the product. ImmuQuest will also need a VAT number for customers in the European Union.UK customers that are VAT exempt need to fax an exemption certificate.

Orders can be placed either by our website, via email or by mail. Please see our contact us page for more details. All orders are subject to availability. Prices of products do not include shipping, VAT or import duties where these are applicable.

Price and other information provided are subject to change without notice. While every effort is made to keep information provided Up to date, ImmuQuest will not be liable if errors should occur in such information. Formal acceptance of an order will take place when the goods are dispatched. If prices should be changed between the time of receipt of an order and dispatch, ImmuQuest will contact you in advance.